Measurement and Analysis of Kinetic Isotope Effects (Volume 596) (Methods in Enzymology, Volume 596)

Author: Harris, Michael E.

Brand: Academic Press

Edition: 1

ISBN: 9780128122730

Release Date: 27-09-2017

Package Dimensions: 9.1 x 6.3 x 1.4 inches

Languages: English

Binding: Hardcover

Number Of Pages: 632

Details: Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD.
Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions. Written by pioneers of modern isotope effect research Is the only collection of modern kinetic isotope effect methods currently available