Apobec Enzymes (Volume 713) (Methods in Enzymology, Volume 713)

Author: Papavasiliou, F. Nina

Edition: 1

ISBN: 9780443317866

Release Date: 01-04-2025

Package Dimensions: 9.7 x 6.9 x 0.9 inches

Languages: English

Binding: Hardcover

Number Of Pages: 328

Details: Apobec Enzymes, Volume 713 in this series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Fluorescent shift assay for APOBECs RNA editing, Low Error Sequencing Methods to Detect APOBEC-mediated RNA editing: Circular RNAseq and Safe-Sequencing System, “Safe-Barcode” RNAseq assay for APOBECs RNA editing, DT40 cell system to characterize somatic hypermutation, CH12 cell system to assay AID activity on class switch recombination, Purification of Enzymatically Active APOBEC Proteins from an Insect Cell Expression System, and more.

Additional chapters cover Defining genome-wide mutagenic impact of APOBEC3 enzymes, APOBEC-induced mutational assay in yeast, Assays for APOBEC drug discovery, Biochemical assay for the identification of APOBECs inhibitors, An In Vitro Cytidine Deaminase Assay to Monitor APOBEC activity on DNA, Profiling rare C-to-U editing events via direct RNA sequencing, Global quantification of off-target activity by base editors, and so much more. Provides a thorough introduction to concepts surrounding circadian rhythms, including their biological basis Incorporates insights from various disciplines, such as biology, medicines, Psychology, and Neuroscience Addresses possible research directions and advancements in the field of circadian rhythms